Figure 1-4264.

Northern blotting analysis of MMP-12 mRNA tissue distribution in Tg and control rabbits. A: MMP-12 mRNA expression is increased in Tg rabbits compared to control rabbits. Rehybridization of the membrane with a human β-actin probe showed that similar amounts of RNA had been loaded in each lane. B: To quantitate the relative elevation of MMP-12 expression, the bands of MMP-12 and β-actin were scanned using a densitometry scanner, and the ratio of MMP-12 to β-actin was calculated. Total RNA isolated from human U937 cell-derived macrophages was used as a positive control.10C: Specific expression of hMMP-12 was demonstrated in the lung, liver, spleen, and bone marrow in Tg rabbits by RT-PCR. D: Increased MMP-12 expression levels in macrophages of Tg rabbits were shown by real-time RT-PCR analysis. N = 3 for each group; *, P < 0.01 versus control