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. 2004 Oct;165(4):1315–1329. doi: 10.1016/S0002-9440(10)63390-1

Figure 3-4260.

Figure 3-4260

Ectopic expression of E-cadherin rescues E-cadherin-negative cells from anoikis. A: FACS analysis of E-cadherin-transfected C8 (C8/E-cad) cells. E-cadherin-negative C8 cells are shown as a control. B: C8/E-cad cells were cultured as MCAs for 6 hours and then transferred onto glass coverslips and immunostained for EGFR (green), and E-cadherin or activated EGFR (p-EGFR) (red). C: Immunoblot analysis of activated EGFR in E-cadherin-transfected C8 MCAs at 24 hours. E-cadherin-positive D1 and E-cadherin-negative C8 MCAs are shown as controls. D: TUNEL analysis of E-cadherin-transfected C8 MCAs at 48 hours. D1 and C8 MCAs are shown as controls.