Skip to main content
. 2004 Oct;165(4):1315–1329. doi: 10.1016/S0002-9440(10)63390-1

Figure 4-4260.

Figure 4-4260

EGFR-dependent ERK/MAPK activation in HSC-3 cell aggregates. A: ERK1/2-MAPK activation pattern in HSC-3 MCAs was determined as described in Experimental Procedures. B: Effect of AG1478 on ERK1/2 phosphorylation. HSC-3 cells were plated as MCA culture in the absence or presence of 1 μmol/L AG1478 for 24 hours before protein extraction. The MEK inhibitor U0126 at 1 and 10 μmol/L was used as the control. C: TUNEL analysis of 10 μmol/L U0126-treated or 50 μmol/L LY294002-treated HSC-3 MCAs at 48 hours as described in Experimental Procedures. D: Akt activation pattern in HSC-3 MCAs using specific antibodies. E: DNA fragmentation of HSC-3 cells. HSC-3 cells were plated as MCA culture in the presence of 50 μmol/L LY294002, 10 μmol/L U0126, or 1 μg/ml AG1478 for 48 hours.