Whole lung CCL17 and CCL22 protein levels before and at days 4 and 8 after S. mansoni egg infusion into S. mansoni-sensitized mice. Whole lung samples were processed as described in the Materials and Methods section, and ELISA was used to determine specific chemokine levels in cell-free supernatants. Data are mean ± SEM of n = 5 per group. *, P ≤ 0.05 compared with chemokine levels detected in whole lung samples removed from S. mansoni-sensitized mice immediately before egg injection (ie, day 0). τ, P ≤ 0.05 compared with chemokine levels detected in naïve mice (mean ± SEM denoted by solid and dashed lines, respectively).