Figure 3-4247.

Spontaneous CCL17 and CCL22 generation by isolated alveolar macrophages (top) from naïve (ie, normal) and S. mansoni-sensitized and -challenged mice. Spontaneous and cytokine-induced CCL17 generation by cultured pulmonary fibroblasts (bottom) from naïve and S. mansoni-sensitized and -challenged mice. ELISA was used to determine specific chemokine levels in cell-free supernatants removed at 24 hours after culture. Data are mean ± SEM of triplicate or quadruplicate samples. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001 compared with chemokine levels detected in culture wells containing normal alveolar macrophages or pulmonary fibroblasts. τ, P ≤ 0.05 compared with chemokine levels detected in tissue culture wells containing media-treated S. mansoni pulmonary fibroblasts. ϕ, P ≤ 0.05 compared with chemokine levels detected in tissue culture wells containing IL-4- or IL-13-treated S. mansoni pulmonary fibroblasts.