Table 3.

Intra-Exon 2 Specificity, MHC Restriction, and TCR V_β Usage of Exon 2-Specific T Cells

T cell line or clone (origin)	Exon 2 epitope	MHC restriction‡	TCR Vβ
line 3 (GKO)	aa 1–26*	I-Ad	Vβ2 (>90%)
		I-Ed	Vβ7 (<5%)
3a.56 (GKO)	aa 1–26*	I-Ad	Vβ2
X2.502 (GKO)	aa 14–26	I-Ed	Vβ8.3
X2.51 (GKO)	aa 14–26	I-Ed	Vβ8.3
BC.D9 (BALB)	aa 6–20	I-Ad	Vβ8.2
6-G.10 (BALB−shi/shi)	aa 14–26	I-Ed	Vβ1†

^*Amino acids 1–26 comprise the entire exon 2 peptide; these cells do not react with peptides comprised of amino acids 1–15, 1–13, 6–20, 11–26, or 14–26. 

^†Identified by RT-PCR; all others identified by flow cytometry. 

^‡Determined by blocking antigen-specific proliferation with antibodies to either I-A^d (34-5-3) or I-E^d (14-4-4).