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. 2004 Nov;165(5):1499–1508. doi: 10.1016/S0002-9440(10)63408-6

Figure 5.

Figure 5

The effect of HCV core on insulin signaling molecules. a: The effect of HCV core on IRS1, IRS2, SOCS3, and insulin receptor expression. Myc-tagged HCV core was transiently expressed in HepG2 cells. Twenty-four hours after transfection the cell extracts were immunoblotted with the indicated antibodies. MG132 (10 μmol/L) was added with cDNA of Myc-tagged HCV core and incubated for 1 hour. STAT5 was used as a reference protein. b and c: The identification of ubiquitinated IRS1 and IRS2. Whole-cell extracts (40 μg of crude extract) were subjected to immunoprecipitation with IRS1 or IRS2 and followed by immunoblotting using anti-ubiquitin monoclonal antibody. d: The effect of HCV core on IRS1 and IRS2 expression in SOCS3−/− MEF cells. Twenty-four hours after transfection of HCV core, MEF cell extracts were immunoblotted with anti-IRS1 antibodies or anti-IRS2 antibodies. Representative immunoblotting images from three separate experiments are shown.