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. 2004 Nov;165(5):1499–1508. doi: 10.1016/S0002-9440(10)63408-6

Figure 6.

Figure 6

The effects of HCV-core on insulin signaling. HLF cells with transfection of empty vector or Myc-HCV core were incubated in the presence of insulin (100 ng/ml) for 60 minutes. a: The effect of HCV-core on insulin-induced phosphorylation of p85 subunit of phosphatidylinositol 3-kinase (PI3K) and Akt. Whole-cell lysates were subjected to immunoblotting for phospho-p85 subunit of PI3K and phospho-Akt. STAT5 was used as reference protein. Representative immunoblotting images from three separate experiments are shown. b: The effect of HCV-core on insulin-induced activation of Fru 6-P, 2-kinase. Sixty minutes after insulin stimulation, Fru 6-P, 2-kinase activity in whole-cell lysates was assayed. Values were expressed as mean ± SD. The comparison between the two groups was made using the Mann-Whitney U-test. *, P < 0.05. c: The effect of HCV-core on insulin-induced activation of glucose uptake. Glucose levels in culture medium were measured at 0, 30, and 60 minutes after insulin stimulation. Values were expressed as mean ± SD. The comparisons between groups were made using analysis of variance with Scheffé’s post hoc test. N.S., not significant. *, P < 0.05.