Figure 7.

Loss of CnA-α results in increased p27 expression in the NZ. A: p27 protein expression was examined in whole kidney homogenates by direct immunoblotting. Each lane represents one animal. Actin was also detected as a control for equal loading. B: p27 expression was determined by immunohistochemistry in wild-type and CnA-α −/− mice using a specific antibody. Protein expression was detected with DAB chromagen and sections were counter-stained with hemotoxylin. The nephrogenic zone (NZ) is indicated in brackets. (i) Four-day-old wild-type animal showing normal p27 expression, (ii) Four-day-old CnA-α −/− animal showing increased p27 expression, (iii) higher magnification of wild-type cortical section showing normal p27 expression in glomeruli, and (iv) higher magnification of CnA-α −/− cortical section showing increased p27 expression in glomeruli. Results are representative of at least three animals of each genotype.