Table 1.
Primers for RT-PCR*
Primer’s name | Oligonucleotide sequence | Product size | |
---|---|---|---|
ADAM8 | Forward | 5′-GCCGTCTTCAGGCCTCGGCCCGGGGACTCT-3′ | 651 bp |
Reverse | 5′-AGGGGCGTTGGCGAGGCACACCGACTGCGG-3′ | ||
ADAM9 | Forward | 5′-GCTGTCTTGCCACAGACCCGGTATGTGGAG-3′ | 604 bp |
Reverse | 5′-TGGAATATTAAGAAGGCAGTTTCCTCCTTT-3′ | ||
ADAM10 | Forward | 5′-ATCCAGTCATGTTAAAGCGATTGATACAATTTAC-3′ | 434 bp |
Reverse | 5′-TCCAAAGTTATGTCCAACTTCGTGAGCAAAAGTAA-3′ | ||
ADAM12s | Forward | 5′-GCACCTCCCTTCTGTGACAAGTTT-3′ | 504 bp |
Reverse | 5′-TGAAAGGCCAGACTTTTGAGTCCT-3′ | ||
ADAM12m | Forward | 5′-GCACCTCCCTTCTGTGACAAGTTT-3′ | 643 bp |
Reverse | 5′-CTTGGTGTGGATATTGTGGAGCAG-3′ | ||
ADAM15 | Forward | 5′-CTGGGACAGCGCCACATTCGCCGGAGGCGG-3′ | 688 bp |
Reverse | 5′-TCCGCAGAAAGCAGCCATAGGGGGTAGGCT-3′ | ||
ADAM17 | Forward | 5′-AGAGCTGACCCAGATCCCATGAAGAACACG-3′ | 777 bp |
Reverse | 5′-GCGTTCTTGAAAACACTCCTGGGCCTTACT-3′ | ||
ADAM19 | Forward | 5′-TGTGGGAAGATCCAGTGTCA-3′ | 500 bp |
Reverse | 5′-AGAGCTGAGGGCTTGAGTTG-3′ | ||
ADAM20 | Forward | 5′-AAAATAGCACACCAGATGGAGTTGCAATTG-3′ | 702 bp |
Reverse | 5′-ATTCCCACAGTACTTCAGTCTAAATATATT-3′ | ||
ADAM21 | Forward | 5′-TCTGGCTTGGGGTATTTTTG-3′ | 500 bp |
Reverse | 5′-TTGGCGTGCTACTTCCTTCT-3′ | ||
ADAM28s | Forward | 5′-GCTGTGATGCTAAGACATGT-3′ | 644 bp |
Reverse | 5′-GTTTATGATCTTAGTAGGGTTGCC-3′ | ||
ADAM28m | Forward | 5′-GCTGTGATGCTAAGACATGT-3′ | 871 bp |
Reverse | 5′-TGAACAGCCTTTACCATCTG-3′ | ||
ADAM30 | Forward | 5′-AACCAGGTGCCAACTGTAGC-3′ | 496 bp |
Reverse | 5′-CCCATGGGTTTCATGGATAG-3′ | ||
HB-EGF | Forward | 5′-ACAAGCACTGGCCACACCAAACAAG-3′ | 299 bp |
Reverse | 5′-CCACGATGACCAGCAGACAGACAGA-3′ | ||
β-actin | Forward | 5′-TGACGGGGTCACCCACACTGTGCCCATCTA-3′ | 661 bp |
Reverse | 5′-CTAGAAGCATTTGCGGTGGACGATGGAGGG-3′ |
Primers for ADAM species, HB-EGF, and β-actin. The estimated product sizes are shown in this setting.