Figure 1.

CEACAM1 expression enhances cell invasion and cell migration. A: Matrigel invasion assay. B: Boyden chamber assay. Cells (2.5 × 10⁴) within passages 6 to 10 were seeded in the upper compartment of transwell chambers containing PET filters precoated with Matrigel (A) or polyester filters precoated with collagen I (B). After 24 hours (invasion assay) or 5 hours (migration assay), under serum-free conditions (RPMI), cells that invaded through the 8-μm pores were stained. The data are means ± SD of five separate experiments. The mean invasion of untransfected MV3 or untransfected MEL6 cells was assigned a value of 1.