Table 1.
siRNAs for Renilla luciferase knockdown
| siRNA | Design target | siRNA sequence |
|---|---|---|
| Student siRNA-1 | R_luc bp 1–156 | CUACUAUGAUUCCGAGAAGdTdTdTdTGAUGAUACUAAGGCUCUUC |
| Student siRNA-2 | R_luc bp 157–312 | UGGGUAAGUCCGGCAAGAGdTdTdTdTACCCAUUCAGGCCGUUCUC |
| Student siRNA-3 | R_luc bp 313–468 | GAUCAAGGCCAUCGUCCAUdTdTdTdTCUAGUUCCGGUAGCAGGUA |
| Student siRNA-4 | R_luc bp 469–624 | UAACUUCUUCGUCGAGACCdTdTdTdTAUUGAAGAAGCAGCUCUGG |
| Student siRNA-5 | R_luc bp 625–780 | CUACAACGCCUACCUUCGGdTdTdTdTGAUGUUGCGGAUGGAAGCC |
| Student siRNA-6 | R_luc bp 781–936 | GUACAUCAAGAGCUUCGUGdTdTdTdTGAUGUAGUUCUCGAAGCAC |
| Validated siRNA | Promega | GGCCUUUCACUACUCCUACdTdTdTdTCCGGAAAGUGAUGAGGAUGA |
| Nontargeting siRNA | Dharmacon RNA Technologies (Lafayette, CO) catalog no. D-001210-02-05 | UAAGGCUAUGAAGAGAUACdTdTdTdTAUUCCGAUACUUCUCUAUGA |