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. 2004 Dec;165(6):1931–1941. doi: 10.1016/S0002-9440(10)63245-2

Figure 2.

Figure 2

Three-dimensional EIII8-HUVEC co-cultures show higher steady-state levels of secreted galectin-3 and differences in protease sensitivity between secreted and intracellular galectin-3. Proteins, 20 or 50 μg, present in culture media and corresponding matrix fractions, respectively, at day 5 were analyzed by Western blotting. A and B: Galectin-3 levels in conditioned media of EIII8 or EIII8-HUVEC co-cultures that were untreated (control) or treated with 0.01 or 0.1% CP or MCP. A’ and B’: Galectin-3 detected in lysates of untreated, CP- or MCP-treated EIII8-HUVEC co-cultures, and EIII8 homotypic cultures. A and A’: Galectin-3 detected with polyclonal HL-31. B and B’: Detection with monoclonal TIB-166 galectin-3 antibody. A“: Short exposure of A’. C: Coomassie blue R250 staining of proteins in conditioned media; C’: Steady-state levels of β-actin in lysate samples. Arrows indicate positions of 62 (A)-, 27 (A)-, and 22 (A’)-kd bands.