Figure 4.
High IL-10 concentrations can be detected in supernatants from metastatic melanoma cultures. To measure IL-10 concentrations in supernatants from primary as well as metastatic melanoma cell cultures (1, 2, and >2 passages) and normal fibroblasts, 106 cells in 25-cm2 culture flasks were cultured in 10 ml of complete RPMI for 24 hours. Supernatants were thereafter centrifuged to remove debris and the IL-10 concentration was assessed by ELISA. MM, metastatic melanoma; PM, primary melanoma; P, passage. *, P < 0.05, in relation to first passage metastatic melanoma IL-10 concentrations in supernatants; **, P < 0.05, in relation to second passage metastatic melanoma IL-10 concentrations in supernatants.