Figure 7.

CD1 down-regulation on monocyte-derived DC is IL-10 mediated. Monocyte-derived DC were generated in the presence of IL-4 (1000 IU/ml) and GM-CSF (800 IU/ml). After 3 days, 50% of the culture medium was replaced with different melanoma cell culture supernatants containing various IL-10 concentrations (A: Cell culture 000630, 306 pg/ml IL-10. B: Cell culture 000622, 26.9 pg/ml IL-10), which have been pre-incubated with a range of concentrations of an IL-10-neutralizing antibody (0, 0.1, 1, and 10 μg/ml) or a corresponding isotype-matched control antibody (IgG_2b, 10 μg/ml). DC cultured in the absence of melanoma supernatant were used as control. CD1a, b, and c expression was measured thereafter by flow cytometry. Results are shown as the mean MFI of three independent experiments. *, P < 0.05, in relation to monocyte-derived DC cultured in the presence of 50% cell culture supernatant in the absence of an antibody.