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. 2004 Dec;165(6):2033–2043. doi: 10.1016/s0002-9440(10)63254-3

Figure 5.

Figure 5

AGE-induced fibrotic effect in the primary cultures of rat MCs. Fibrotic effects and FN and Col IV overproduction were measured in the MCs in vitro directly exposed to AGEs, as described in Material and Methods. A and C: The dose-response of AGEs (48 hours) on FN and Col IV overproduction. B and D: The time-response of AGEs (100 μg/ml) on FN and Col IV overproduction. E: The effect of CTGF neutralization on AGE-induced overproduction of FN and Col IV. In the AGE-BSA group, MCs were exposed to 100 μg/ml of AGE-BSA for 48 hours. In the anti-CTGF group, MCs were pretreated with goat anti-CTGF (10 μg/ml) for 2 hours and then co-incubated with 100 μg/ml of AGE-BSA for 48 hours. *, P < 0.05 versus control; #, P < 0.05 versus AGE-BSA.