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. 2004 Dec;165(6):2033–2043. doi: 10.1016/s0002-9440(10)63254-3

Figure 7.

Figure 7

Effect of silencing TGF-β1 mRNA expression or neutralization of TGF-β1 protein on AGE-induced CTGF expression. MCs were treated with shRNA for the indicated times and TGF-β1 mRNA expression was analyzed by RT-PCR, as described in Material and Methods. TGF-β1 mRNA expression in MCs was significantly inhibited starting at 24 hours and reaching maximal levels at 48 hours after incubation with TGF-β1 shRNA, but not changed until 48 hours after incubation with luciferase shRNA (shRNA-luc) (A); therefore, MCs were incubated with TGF-β1 shRNA for 24 hours and then added with 100 μg/ml of AGEs for another 24 hours (total 48 hours for TGF-β1 shRNA incubation). Expression of TGF-β1, CTGF, and GAPDH mRNA was analyzed by RT-PCR (B). C: The results of AGE- or TGF-β1-induced CTGF mRNA expression with and without neutralizing AGEs or TGF-β1 protein by anti-AGE or anti-TGF-β1 neutralizing antibody. In this experiment, MCs were treated with anti-AGE antibody (100 μg/ml) and anti-TGF-β1 antibody (10 μg/ml) for 2 hours before exposure to AGEs (100 μg/ml) or TGF-β1 (10 ng/ml) for 48 hours. *, P < 0.05 versus control; #, P < 0.05 versus AGE-BSA.