Table 1.
Liver Granuloma Formation in L. donovani-Infected Mice at Day 14 after Infection*
| Tissue response | Percent infected foci showing the indicated cellular response†
|
|||||
|---|---|---|---|---|---|---|
| C57BL/6 | B6.TNFα−/− | B6.LTα−/− | B6 →B6.CD45.1 | B6.TNFα−/− →B6.CD45.1 | B6.LTα−/− →B6.CD45.1 | |
| KC | 19.7 ± 2.0 | 41 ± 2.0‡ | 22.2 ± 0.4‡ | 24.7 ± 2.1 | 25.9 ± 3.5 | 19.9 ± 1.7§ |
| IG | 57 ± 1.5 | 59 ± 1.8 | 74.4 ± 0.9 | 37.5 ± 5.9 | 47.4 ± 1.8 | 61.8 ± 1.4 |
| MG | 22 ± 3.0 | 0.2 ± 0.2 | 3.4 ± 0.4 | 35.6 ± 3.1 | 26.7 ± 1.8 | 18.3 ± 3.0 |
| SG | 1.1 ± 0.4 | 0 | 0 | 2.2 ± 1.0 | 0 | 0 |
| Total no. infected foci per 25 fields | 233 ± 5.3 | 360 ± 6.4¶ | 345 ± 11.6¶ | 182 ± 3.2 | 240 ± 18.5∥ | 232 ± 12.4∥ |
*
Granuloma counts performed on liver cryosections stained with hamster anti-L. donovani (LV9) Ab, then counterstained with hemotoxylin.
†
KC, infected KC with no surrounding leukocytes; IG, infected KC surrounded by some leukocytes, but not completely; MG, infected KC completely surrounded by leukocyte mantle; SG, sterile (empty) granuloma.
‡§
The distribution of tissue responses is significantly different (P < 0.001) than that observed in L. donovani-infected C57BL/6‡ and B6 → B6.CD45.1§ mice.
¶∥
The number of infected foci is significantly different (P < 0.001¶ and P < 0.05∥) than that observed in L. donovani-infected C57BL/6¶ and B6 → B6.CD45.1∥ mice.