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Profiles generated by single-step MAS-PCR assay with purified DNA preparations from clinical M. tuberculosis strains targeting three rpoB codons: codon 531 (A), codon 526 (B), and codon 516 (C). Lanes: 1, H37Rv strain; 2 and 3, strains with rpoB531 mutant alleles (TCG and TTG); 4 to 6, strains with rpoB526 mutant alleles (GAC, TAC, and CTC); 7 to 9, strains with rpoB516 mutant alleles (GTC, TAC, and GGC); M, 100-bp DNA ladder (Amersham Bioscience).
