TABLE 3.

Characteristics of pACM1 and plasmids from ESBL-producing K. pneumoniae donors with non-pACM1-like hybridization patterns^a

Plasmid	Hybridization pattern	Size (kb)	Restriction fragment fingerprintb	Homology to pACM1 near SHV gene (kb)c	β-Lactamase pId	MIC (μg/ml) of CAZe	Transconjugants per donorf
pACM1	Y6	88	A-1	=	8.2	16	4.1 × 10−4
pACM140	N16	55	A-2	55	8.2	16	7.4 × 10−4
pACM170	N22	35	B	2.5	8.2	32	None
pACM150g	N29	160	C	5.8	5.4, 8.2	6	1.3 × 10−6
pACM120	N29	70	D	7.5	5.4, 8.2	12	None

^aAfter transformation of E. coli DH5α.

^bEcoRI and additional enzymes used.

^c=, not applicable.

^dApproximate pI.

^eCAZ, ceftazidime. MIC for the DH5α host is 0.125 μg/ml.

^fFrom E. coli DH5α donor to DH5α(pACYC184) recipient.

^gFrom clone A outbreak strain.