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. Author manuscript; available in PMC: 2006 Oct 20.
Published in final edited form as: Hum Gene Ther. 2005 Sep;16(9):1110–1115. doi: 10.1089/hum.2005.16.1110

FIG. 1.

FIG. 1

The induction of RNAi against GFP in the human lymphoblastoid .45 cell line using the pseudovirion delivery of plasmids expressing short hairpin RNAs. .45 human lymphoblastoid cells were transduced with a half reaction (A) or with 1/7 reaction (B) of GFP plasmid DNA packaged in vitro only (green). The same cells were transduced with GFP plasmid DNA packaged in vitro and then immediately with a half reaction (A) or with 6/7 reaction (B) IVP-shGFP pSilencer 3.1-H plasmid DNA packaged in vitro (blue), and were compared to mock, empty virion-transduced cells (red). The blue curve appears shifted to the left as compared to the green curve, indicating complete shutdown of the GFP expression.