TABLE 3.
Effect of boiling and proteinase exposure and addition of electron donors and inhibitors of electron transport on the rate of malachite green decolorization by the membrane fraction of M. avium strain A5
| Treatment | Malachite green decolorization rate (%)a ± SD |
|---|---|
| None | 100 |
| Boiling (10 min at 100°C) | <2 ± 0.5 |
| Pronase (1 mg for 60 min at 37°C) | <2 ± 0.2 |
| Fe3+ (1 mM) | 110 ± 15 |
| Fe2+ (1 mM) | 157 ± 21 |
| NADPH (1 mM) | 90 ± 5 |
| NADH (1 mM) | 135 ± 12 |
| NaCN (10 mM) | 116 ± 7 |
| NaN3 (10 mM) | 108 ± 5 |
| DNP (10 mM) | <3 ± 0.5 |
| Metyrapone (10 mM) | <3 ± 0.8 |
a
Average of three separate measurements of two different membrane fractions.