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Concentration of 40 mM d-glucose decreases phospho-phosphatidylinositol 3-kinase (PI3k) expression in ECs. Immunoblots were performed for tyrosine-phosphorylated proteins after immunoprecipitation of PI3k from lysates obtained from HUVEC cultured in 5, 20, and 40 mM d-glucose. Total PI3k was evaluated by reprobing membranes. Bar graph shows the mean relative band intensity ± SE of tyrosine-phosphorylated PI3k in lysates. *P < 0.05 compared with control (5 mM d-glucose).
