Table 2.

Inactivation of HIV-1 IIIB and BaL by formulated Aquateric (18%) (5 min, 37°C) in the presence of seminal plasma

Seminal Plasma: Formula Ratio		% Virus Inactivation
		HIV-1 IIIB		HIV-1 BaL
Ratio	pH	Aquateric Pellet	Supernatant	Aquateric Pellet	Supernatant
0.33	3.9	≥ 99.8	≥ 99.8	≥ 99.6	≥ 99.6
1.0	4.8	≥ 99.8	≥ 99.8	≥ 99.6	≥ 99.6
3.0	5.43	≥ 99.8	≥ 99.8	≥ 99.6	≥ 99.6

Virus concentrates of HIV-1 IIIB and Bal, respectively, were diluted 200-fold in seminal plasma and then mixed with Aquateric formulation 1 (see Methods section) at volume ratios indicated in the Table. After incubation for 5 min at 37°C, the samples were cooled in ice and centrifuged at 10,000 rpm for 10 min. The supernatant fluids were neutralized by addition of 1 M Na₂HPO₄. The pellets containing Aquateric with adsorbed virus were resuspended and brought to pH 7.0 by addition of 1 M Na₂HPO₄. Both the supernatants and the redissolved pellets were mixed with a solution of PEG 8000 (final concentration 3%). The resulting pellets were resuspended in DMEM medium and tested for infectivity as described in the Methods Section. Virus preparations diluted in seminal fluid but not treated with the Aquateric formulation represented controls.