Table 1.
Cassette/Template | Primer sequence |
CCNA2-SOM1/336M6 | 5'-GTTCTAGACTGCTTTGTTTGCCCATTTTTCTC |
3'-CTTGGATCCCTCCCCACTGTACAATAAAACAA | |
CCNA2-SOM2/336M6 | 5'-AATTTCTAGAGGTTAACATTGGTGCTGCCAAC |
3'-GTTTGGATCCCCCTGAGCAATGAAAGTGGAC | |
CCNA2-GC1/336M6 | 5'-GTCTAGAGTAGGAAAAGATTTTATAAAAC |
3'-CATGGATCCCTGTAACATCTGAGAGCCGATC | |
CCNA2-GC2/336M6 | 5'-CTCTAGAGAATCTTACCCTGTTCTAAGTAAG |
3'-CGGATCCCATGGGCAGTCCTGGTAGCAAG | |
CREM-somatic/304K8 | 5'-TCTAGAGTCACGTAGGTCTAGACTGGC |
3'-GGATCCCTGAGTTCGGATCCCAACACC | |
CREM-MGC/304K8 | 5'-TCTAGAAACAAGAAGCTTCTAGAGGAGC |
3'-GGATCCCCTTAGAGGTAGGATCCAATTAAATAC | |
c-abl-somatic/156H9 | 5'-TCTAGACCAGTACATTTTGTTTCTAGATATGACTG |
3'-GGATCCCTGCTGTGAGGATCCCAAGAG | |
c-abl-mgc/156H9 | 5'-TCTAGATTCCCTGTCCTTCTAGAAC |
3'-GGATCCGATGGACCTTCTAGACACCAAG |
Shown at left are the names of the polyadenylation cassettes with the BAC template used as a template to create each cassette, and at right the sequences of the primers used to make the respective polyadenylation cassette. The XbaI and BamHI restriction enzyme sites used for cloning are shown in bold and italics, respectively.