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. Author manuscript; available in PMC: 2007 Jul 1.
Published in final edited form as: Epilepsy Res. 2006 Mar 20;70(1):3–14. doi: 10.1016/j.eplepsyres.2006.02.002

Figure 4.

Figure 4

Immunoprecipitation of active caspases reveals caspase 8 is activated following seizure. Cells were incubated with biotin-zVAD-fmk (1 μM) and subject to seizures and 4 h recovery. Bound caspases were precipitated with avidin-agarose and immunoblotted for caspase 2, 3,8 9 and 12. As a positive control cortical brain lysate from 100 min MCAO treated rats was used (see (Meller, et al. 2005)). Note lack of cleaved caspase 2 9 and 12, and the increase in cleaved caspase 8, 4 hours following seizure. Caspase 3 was also cleaved following seizure. Images shown are representative blots from 3 separate experiments with identical results. Numbers at the side of the blots represent molecular weight in kDa.