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. Author manuscript; available in PMC: 2007 Sep 15.
Published in final edited form as: Anal Biochem. 2006 Jul 10;356(2):222–228. doi: 10.1016/j.ab.2006.06.028

Figure 3.

Figure 3

Products obtained using current and improved 3′-RACE methods with an extremely GC-rich gene. The results obtained with a representative “standard” 3′-RACE method described in Materials and methods are shown in lanes 1 (UPM primer control), 2 (UPM+TniFucT11), and 3 (TniFucT11 single primer control). The results obtained using the improved 3′-RACE method described in Materials and methods are shown in lanes 4 (UPM primer control), 5 (UPM+TniFucT11), and 6 (TniFucT11 single primer control). The specific TnFT3 3′-RACE product is marked by the arrowhead. The lane marked M contains a 1 kb and 100 bp DNA ladders (New England Biolabs).