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. 2000 Mar 28;97(7):3056–3061. doi: 10.1073/pnas.97.7.3056

Figure 4.

Figure 4

UTP misincorporation by wild-type (WT) and mutant HIV-1 RTs. An unlabeled sequencing primer was annealed to single-strand M13mp18 DNA. RT polymerization was done with radioactive [α32P]UTP in the reaction mixture. Extension products with UTP incorporation were radioactively labeled and visible on x-ray film. Duplicate assays, labeled 1 and 2, were done for each sample.