Figure 5.

Inhibition curves for various nucleoside analogs. The amount of radioactivity incorporated into the template-primer by wild-type and mutant HIV-1 RTs in the absence of inhibitor represents 100% enzyme activity. The amount of radioactivity incorporated at the various concentration of inhibitor is normalized to this value. The template primer for testing ddITP and ddGTP inhibition was poly(rC)⋅oligo(dG), whereas that for ddTTP and AZTTP was poly(rA)⋅oligo(dT). The template-primer for testing (−)-β-l-2′,3′-dideoxy-3′-thiacytidine triphosphate (3TCTP) inhibition was a sequencing primer annealed to single-strand M13mp18 DNA. When single-strand M13mp18 was used as a template with the −47 primer, the Tyr-115–Val mutant produced 36% as much DNA product as wild-type HIV-1 RT; with poly(rA)⋅oligo(dT) and poly(rC)⋅oligo(dT), the Tyr-115–Val mutant produced 50–75% as much DNA product.