FIG. 4.

Sequences and structures around SL2 are required for the differential inhibitory effects of HDAg-S and HDAg-L on genotype III amber/W editing. Huh-7 cells were cotransfected with the 500 ng of the nonreplicating genotype III antigenomic RNA expression construct pHDV-III-NR containing the indicated stem-loop deletion mutations and indicated amounts of genotype III HDAg-S or HDAg-L expression constructs. Editing was analyzed as in Fig. 1. Symbols: ▪, HDAg-S; □, HDAg-L. Also indicated on the right are schematic diagrams of the effects of the mutations on the predicted structure required for editing. Elements of schematic diagrams are as in Fig. 3; deleted sequences are shown by a dotted line. The mutations analyzed were as follows: ΔSL1-M24, top panel; ΔSL2-M25, middle panel; and ΔSL1,ΔSL2-M24/25, bottom panel.