FIG. 1.
Detection of PrPSc in brain tissue of wild-type mice with subclinical prion disease orally inoculated with RML 5.0 prions. Aliquots of 10% (wt/vol) whole-brain homogenates (wild-type mice) or brain stems (tga20 mice) from mice that had succumbed to terminal prion disease (i.c. or i.p. inoculated) or orally inoculated mice that were euthanized either 375 or 525 days after prion inoculation were treated with PK and subjected to Western blotting with rabbit polyclonal anti-PrP serum XN and enhanced chemiluminescence. Sample portions (10 μl for non-PK-treated samples and 15 μl for PK-treated samples) (equivalent to 40 to 50 μg of total protein) were loaded on the gel. The positions of molecular mass markers (in kilodaltons) are indicated to the left of the gels. Samples were digested without (−) or with (+) PK.