TABLE 1.
Incubation time for prion disease in wild-type and tga20 mice inoculated with RML 5.0 prions by different routesa
| Inoculation route | Wild-type mice
|
tga20 mice
|
||
|---|---|---|---|---|
| Diseaseb | Incubationc | Disease | Incubation | |
| i.c. | 5/5 | 151 ± 9 | 5/5 | 67 ± 2 |
| 5/5 | 153 ± 0 | 5/5 | 73 ± 8 | |
| i.p. | 5/5 | 225 ± 0 | 5/5 | 130 ± 19 |
| 5/5 | 204 ± 10 | 5/5 | 113 ± 3 | |
| Oral | 0/3 | >600 | 5/5 | 168 ± 10 |
| 0/3 | >600 | 5/5 | 140 ± 13 | |
a
Wild-type and tga20 mice were inoculated by the i.c., i.p., or oral route with 1.32 × 105 LD50 of RML 5.0. Inoculated mice were monitored daily for clinical signs of mouse prion disease. Mice that succumbed to prion disease were euthanized at the point of neurological disease and dysfunction.
b
Number of mice succumbing to terminal disease/number of mice in each group.
c
Mean time to terminal prion disease (in days) ± standard deviation.