FIG. 1.
Schematic representation of viral cosmids and plasmids used to generate HSV-1 recombinants. (A) Inserts of the five cosmids (24, 32, 48, 51, and 71) covering the complete genome of the HSV-1 strain 17 (16), along with the three plasmids (pNEB23, pPol6, and pNEB10) designed to replace cos71. Tr, terminal repeat; UL, unique long; US, unique short; Ir, internal repeat. (B) Construction of the pNEB23, pPol6, and pNEB10 plasmids. The nucleotide positions of inserts in the HSV strain 17 genome are indicated.