TABLE 1.

PCR primers used in this study

Primer	Gene region	5′-3′ sequencea	Orientation
GDTD1B	Polymerase	CGGCATGCGACAAACACGGAGTCNGTRTCNCCRTA	− outer
DFASA	Polymerase	GTGTTCGACTTYGCNAGYYTNTAYCC	+ outer
VYGA	Polymerase	ACGTGCAACGCGGTGTAYGGNKTNACNGG	+ inner
PapRV2 S1	Polymerase	GTCACGGTTTCCGCTATGTT	− inner
PapRV2 S2	Polymerase	ACGGTTTCCGCTATGTTGAT	− inner
TS1A	TS	GAGYTGCTKTGGTTTMTSARGGG	+ outer
TS1B	TS	CCCATRTCDSCBGAYCTCTG	− outer
TS2A	TS	GGNTTCCAGTGGAGRCAYTTYGG	+ inner
TS2B	TS	TGRTACAGCTGRCAGGAMAGC	− inner
PapRV2 pol 1-f	Polymerase	GAGCATCATCCAGGCACACA	+ real time
PapRV2 pol 1-r	Polymerase	GTGCAGGTGTAGGTCCCCC	− real time
PapRV2 pol 2-f	Polymerase	CTAGACAAACAGCAGCTGGCC	+ real time
PapRV2 pol 2-r	Polymerase	ACCCCGGTGAACCCGT	− real time
PapRV2 TS-f	TS	TGACGATGCGCCGATC	+ real time
PapRV2 TS-r	TS	AGCTGCGATACGTGATGGA	− real time
CMVpap gB-f	gB	CACAGAGCGGTAAGGTTGGC	+ real time
CMVpap gB-r	gB	TACTCGTTCTCCTCCACCGG	− real time
SV40-f	Large T antigen	GTCTTCTACCTTTCTCTTCTTT	+ real time
SV40-r	Large T antigen	GGAGCAGTGGTGGAA	− real time
Chymase pap-f	Chymase	CCTTCCCATCCCAATTCAACT	+ real time
Chymase pap-r	Chymase	CTGTTCTTCCCCAGCCAG	− real time

^aR = A/G, Y = C/T, M = A/C, K = G/T, S = C/G, B = C/G/T, N = A/C/G/T.