FIG. 1.

Induction of cell death in cells infected with wild-type (wt) BUN or BUNdelNSs. Cells were infected at an MOI of 1 PFU/cell. (A) Shrinkage and death of 2fTGH and 2f/SV5-V at 24 h postinfection. Cells were examined by phase contrast microscopy. (B) DNA fragmentation in 2fTGH and 2f/SV5-V cells at 24 h and 48 h postinfection. Low-molecular-weight DNA was isolated as described under Materials and Methods, and DNA fragmentation was analyzed by electrophoresis in a 1.5% agarose gel followed by ethidium bromide staining.