Figure 4.

Typical electron micrographs of negatively stained TrbB (a) and HP0525 (b) samples in the presence of Mg²⁺ and ATP. The arrrowheads point to some characteristic particles selected from these fields. The bar represents 50 nm. Two galleries with the type of images that were used in the two-dimensional analysis are shown in c (TrbB) and d (HP0525). The global average images are shown in e (TrbB) and f (HP0525) and have been low-pass filtered to their final resolution (14 Å in both cases). The size of the frames of each image in the galleries and for the final average images is 20.0 × 20.0 nm.