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. 2003 Jul;77(14):8147–8152. doi: 10.1128/JVI.77.14.8147-8152.2003

FIG. 3.

FIG. 3.

HHV-8 entry is pH dependent but not clathrin dependent. (A) Procedures to evaluate effects of a 24-h incubation of T1H6 cells in the presence of inhibitors. Beginning time points of 24-h incubation treatments are indicated relative to the time point when HHV-8 was added (0 h). (B) T1H6 cells were infected with HHV-8 and cultured in the presence of 10, 20, and 40 mM ammonium chloride for 24 h; their β-galactosidase activities are shown as %RLU by using the activities obtained from untreated cells as a 100% control. Means and standard deviations from triplicate experiments are shown. (C) T1H6 cells were infected with HHV-8, with LLRN retrovirus vectors pseudotyped with VSV-G (VSV), and with amphotropic MLV envelope (MLV) and cultured in the presence of 40 mM ammonium chloride (C), 12.5 μM bafilomycin A1 (D, left panel), 2 μM monensin (D, middle panel), and 15 μM chlorpromazine (D, right panel). β-Galactosidase activities for HHV-8 infectivity and luciferase activities for the pseudotyped retrovirus vectors on T1H6 cells were measured, and their means and standard deviations for %RLU from triplicate experiments are shown. 293T cells were infected with rSFVβgal (SFV) in the presence of 15 μM chlorpromazine, and the data are shown with those of HHV-8 (D, right panel).