FIG. 4.
Interaction of HIV-1 with HHV-8. (A) Soluble factors from HIV-infected cells had no effect. Conditioned media were prepared from PM-1 cells infected with HIV-1LAI (LAI) (9), HIV-1Ba-L (Ba-L) (23), or HIV-2CDC77618 (HIV-2) (44) or from mock-infected cells, 24 h p.i. T1H6 cells were incubated in the presence of the conditioned media for 24 h and then infected with 0.5 μl of the HHV-8 stock described in the legend for Fig. 1D. The cells were harvested 52 h p.i. β-Galactosidase activities from triplicate experiments are shown as means and standard deviations for %RLU by using the activities obtained from the HHV-8-infected cells without addition of any conditioned media as a 100% control (no sup). (B) HIV-1 expression, but not Tat alone, slightly enhanced HHV-8 replication. T1H6 cells were transfected with vector plasmid pSV2, with pSV-Tat72 (Tat), and with pNL4-3 (HIV FL) and infected with HHV-8 1 day later. They were harvested 52 h p.i. β-Galactosidase activities from each condition are shown as means and standard deviations for %RLU by using the activities obtained from the HHV-8-infected cells that were transfected with pSV2 as a 100% control (shaded bar).