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Single-step growth curves of wild-type and recombinant viruses in BHK-21 cells at 37°C. The cells were infected at a multiplicity of infection of 3, and samples of the supernatant medium were harvested at the indicated time points. The samples were titrated in duplicate by plaque assay on Vero-76 cells. Single-step growth curves comparing VSIV-GNJ and 95COB viruses at early times postinfection are shown in the inset.
