FIG. 2.

Effects of SeV C gene mutations on IFN-β and IL-8 mRNA levels during infection. Parallel cultures of 2C4 cells were infected (or not) in triplicate for 24 h with 20 PFU of the various SeVs per cell. Total cytoplasmic RNA was prepared from each culture, and the same amount of RNA (ca. 1 μg) was transcribed into cDNA with random hexadeoxynucleotides and murine leukemia virus reverse transcriptase. The relative amounts of IFN-β and IL-8 gene sequences, relative to that of GADPH as an internal control, were determined by real-time PCR (see Materials and Methods). The average levels of the mRNAs and their deviations in the triplicate infections are shown. The relative SeV N mRNA levels of the SeV^Z-C-wt and -CΔ10-15 infections were also determined (SeV^Z and SeV^M are 10% different in sequence, and their detection requires different primers and probes).