FIG. 3.

Effects of IRF-3 and dsRNA treatment on SeV-induced host gene activation. Parallel cultures of 2C4 cells were transfected with pRFP or pIRF-3. The cultures were then infected (or not) with 20 PFU of the various SeVs per cell at 20 h posttransfection, and some of the cultures were also treated with 50 μg of poly(I)-poly(C) per ml. The cells were harvested at 24 hpi. The relative amounts of IFN-β, IL-6, and IL-8 gene sequences present were determined as described for Fig. 2.