Table 1.

Primers for the mutagenic reactions

Mutation	Mutagenic oligonucleotide	Mutated codon	Restriction site
MutA/I15T	5′-TCGCGAGTCCTGTGCCTTTATTG-3′	Ile-15 → Thr	NruI
MutB/T15I	5′-TCGCGAGTCCTATGCCTTTATTG-3′	Thr-15 → Ile	NruI
MutB/Q27K	5′-GTAAATTTTGCCTTACAGAGCTC-3′	Gln-27 → Lys	SacI