FIG. 1.
Coimmunoprecipitation and phosphorylation of UL44 and UL97. GST-UL97 (UL97) and His6-UL44 (UL44) were incubated alone or together, as indicated, in kinase buffer in the presence of radiolabeled ATP, in either the presence or absence of 1 μM maribavir. Aliquots of the reaction mixtures were resolved by SDS-PAGE and analyzed with a phosphorimager (lanes 1 to 6). The remaining portions of the reactions were precipitated (IP) (lanes 7 to 16) by the addition of either a UL97-specific antibody (Ab) (lanes 7, 8, 13, and 14), a UL83-specific antibody as a negative control (lanes 9 and 10), or a UL44-specific antibody (lanes 11, 12, 15, and 16), followed by protein A-Sepharose, and resolved by SDS-PAGE and analyzed with a phosphorimager. M, molecular weight markers. Approximately 50% more of each immunoprecipitate was analyzed than the corresponding sample analyzed prior to immunoprecipitation.