FIG. 2.

Purification of UL44. GST-UL44 was expressed in E. coli, the E. coli was lysed, and GST-UL44 was purified initially as a fusion protein, followed by cleavage of the fusion partner, permitting purification of UL44 as detailed in Materials and Methods. Aliquots of the total lysate, the lysate following centrifugation (Soluble lysate), the protein purified on glutathione-Sepharose (Glutathione eluant), the cleaved protein purified on DNA agarose (DNA agarose eluant), and the protein that flowed through a glutathione-Sepharose column (glutathione f.t.) were resolved by SDS-PAGE and detected by Coomassie blue staining. The positions of GST-UL44 and UL44 are indicated to the left.