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In vitro phosphorylation of UL44 by UL97. Recombinant, purified HCMV GST-UL97, GST-UL97 K355Q, and UL44 were incubated alone or together, as indicated, in kinase buffer in the presence of radiolabeled ATP. The proteins were resolved by SDS-PAGE and analyzed with a phosphorimager. The positions of the GST-UL97 and UL44 proteins are indicated to the left gel.
