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. 2003 Jul;77(14):7924–7935. doi: 10.1128/JVI.77.14.7924-7935.2003

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Copyright © 2003, American Society for Microbiology

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FIG. 5. — The Env proteins of JSRV and ENTV are not tyrosine phosphorylated in 208F cells transformed by these proteins. (Left panel) 208F cells transformed by JSRV (Jenv-FLAG) or ENTV (FLAG-Eenv) Env proteins were serum-starved overnight, cell lysates were harvested and were immunoprecipitated (IP) by using anti-FLAG antibody, and immunoprecipitates were immunoblotted (IB) by using antiphosphotyrosine antibody 4G10. Lysate from EGF-stimulated A431 cells (A431/EGF) (Upstate Biotechnology) was run as a positive control. (Right panel) The same PVDF membrane was then stripped and reblotted by using anti-FLAG antibody to ensure that the IP worked properly.