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MAPK phosphorylation was not detected in 208F cells transformed by JSRV or ENTV Env proteins or their tyrosine mutants. Twenty micrograms of each cell lysate was used for SDS-PAGE analysis followed by immunoblotting by using anti-phospho-MAPK or anti-MAPK. pp44 and pp42 represent phospho-Thr202/Tyr204 MAPK, while p44 and p42 represent total cellular MAPK. (A) Cells were serum-starved (DMEM only) overnight. (B) Cells were grown in normal culture medium (DMEM with 5% FBS).
