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. 2000 Mar 14;97(7):3084–3088. doi: 10.1073/pnas.050368997

Figure 2.

Figure 2

A PPN TFO generates mutations in a cognate endogenous target sequence and not in unrelated genes. Number of revertants generated by a PPO TFO, a PPN TFO, or in the absence of TFO (UVA), as indicated, on the HIV-1 target sequence inserted into the endogenous URA3 gene (ura3∷hiv-1), or on irrelevant genes (ade2–101 and lys2–801) of a CmY826-derived strain. TFOs were electroporated into intact yeast cells, cells were irradiated, and URA3+, ADE2+, or LYS2+ revertants were selected on the appropriate medium. Shown is the mean value of three independent experiments. The HIV-1 target sequence is shown in Fig. 4A.