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Northern blot analysis. RNA was isolated from strain UAMS-1 grown to stationary phase (15 h) in planktonic culture (P) or from 1-week-old biofilms generated in a flow cell (B). Equal amounts of RNA (based on A260/A280 ratios) were resolved by agarose electrophoresis and hybridized with probes corresponding to sarA (left panel) or agr (right panel). The specific identity of each transcript is indicated beside each panel.
