FIG. 1.

Western immunoblot reactions of anti-OmpA MAbs. Cell envelopes (or whole cells; see below) from bacterial strains were lysed with SDS by boiling at 100°C for 5 min, subjected to SDS-PAGE, and transferred to nitrocellulose. The paper was cut into strips and incubated with a 1:50 dilution of MAb SH16.2 (lanes 1, 3, 5, and 7). Control strips (lanes 2, 4, 6, and 8) were probed with ascites fluid from the cell fusion partner P3-63-Ag.8.653. The reaction was developed with alkaline phosphatase-labeled goat anti-mouse Ig-nitroblue tetrazolium plus bromochloroindolyl phosphate. Other bacterial strains (whole cells) showing positive reaction patterns included serovar Typhimurium WB600, C. amalonaticus, S. boydii, Klebsiella pneumoniae, Enterobacter cloacae, Serratia marcescens, Edwardsiella tarda, Hafnia alvei, and Yersinia enterocolitica. On the other hand, Proteus vulgaris, Morganella morganii, and Providencia stuartii gave negative reactions.